Use of Alternative Primers for Gender Discrimination in Human Forensic Genotyping

Kupfer, Doris M.; Jenkins, Marita C.; Burian, Dennis; Canfield, Dennis V.

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Use of Alternative Primers for Gender Discrimination in Human Forensic Genotyping

2008-04-01
By Kupfer, Doris M. ; Jenkins, Marita C. ; Burian, Dennis

File Language:

English

Details

Creators:

Kupfer, Doris M. ; Jenkins, Marita C. ; Burian, Dennis ; Canfield, Dennis V.
Corporate Creators:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Corporate Contributors:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Office of Aerospace Medicine
Subject/TRT Terms:
Publication/ Report Number:

DOT/FAA/AM-08/8
Resource Type:

Tech Report
Right Statement:

Public Domain
Geographical Coverage:

United States
Corporate Publisher:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Abstract:

An assay using the Federal Bureau of Investigation's human Combined DNA Identity System (CODIS) primers has been developed for polymerase chain reaction (PCR)-based human identity testing. Recent forensic literature has identified several human populations that carry a deletion mutation in the Y-chromosome copy of the amelogenin locus. This is the standard locus used for gender determination in CODIS. Additionally, the amelogenin male PCR products are very close in size requiring manual annotation of PCR electrophoresis results for this locus. This study was designed to test several gender-specific primers which are to loci outside the amelogenin region, have well-separated PCR products, and could serve as additions or replacements to amelogenin in our human identity testing assay.
Format:

PDF
Download URL:

https://rosap.ntl.bts.gov/view/dot/58334/dot_58334_DS1.pdf
File Type:

[PDF - 714.18 KB]
Collection(s):

FAA Civil Aerospace Medical Institute
Main Document Checksum:

urn:sha256:d1972b79e6c33e2e5794fc02db4e50a002b10c20e55849312ce8f29f1ac7eda9

File Language:

English

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