Testing MiniSTR Primers for Addition to a PCR-Based Forensic Specimen Identification Protocol

Details

• Creators:
  Guzman, Laura ; Kupfer, Doris M. ; Burian, Dennis
• Corporate Creators:
  United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute ; University of Central Oklahoma
• Corporate Contributors:
  United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Office of Aerospace Medicine
• Subject/TRT Terms:
  • Bioassay
  • Forensic medicine
  • Specimens
  • Testing
  • Genotyping
  • Polymerase Chain Reaction
  • miniSTR
• Publication/ Report Number:
  DOT/FAA/AM-09/21
• Resource Type:
  Tech Report
• Geographical Coverage:
  United States
• Corporate Publisher:
  United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
• Abstract:
  An assay has been developed for polymerase chain reaction (PCR)-based human identity testing using the Federal Bureau of Investigation’s human Combined DNA Identity System (CODIS) primers. Recent forensic literature has identified difficulties using these primers due to amplicon size and the degraded nature of DNA from forensic samples. Primers termed mini Short-Tandem Repeat (STR) primers targeted to the same loci as the CODIS primers but which have smaller amplicons have been developed.
• Content Notes:
  Work was accomplished under approved task AM-TOXLAB
• Format:
  PDF
• Download URL:
  https://rosap.ntl.bts.gov/view/dot/57121/dot_57121_DS1.pdf
• File Type:
  [PDF - 480.98 KB]
• Collection(s):
  FAA Civil Aerospace Medical Institute
• Main Document Checksum:
  urn:sha256:61733de1b0de28cce9a26355f34cd8dcaa6f2faf5a8b6c155c9bebe542714a49