A Rapid and Inexpensive PCR-Based STR Genotyping Method for Identifying Forensic Specimens

Kupfer, D. M.; Cassidy, Brandt; Vu, Nicole; Burian, Dennis; Canfield, Dennis V.; Huggins, Mark

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A Rapid and Inexpensive PCR-Based STR Genotyping Method for Identifying Forensic Specimens

2006-06-01
By Kupfer, D. M. ; Cassidy, Brandt ; Vu, Nicole

File Language:

English

Details

Creators:

Kupfer, D. M. ; Cassidy, Brandt ; Vu, Nicole ; Burian, Dennis ; Canfield, Dennis V. ; Huggins, Mark
Corporate Creators:

Advancia Corporation ; DNA Solutions
Corporate Contributors:

Federal Aviation Administration
Subject/TRT Terms:
Publication/ Report Number:

DOT/FAA/AM-06/14
Resource Type:

Tech Report
Geographical Coverage:

United States
Corporate Publisher:

United States. Department of Transportation. Federal Aviation Administration
Abstract:

Situations arise where forensic samples can be inadvertently misidentified during field collection. Samples identified in the field as being from the same person that return conflicting toxicological results may suggest such misidentification. Polymerase chain reaction (PCR)-based human identity testing provides a reliable and independent method to confirm sample identification. In the study presented here, the Federal Bureau of Investigation’s human Combined DNA Identity System (CODIS) loci were used on a rapid, inexpensive microfluidics chip electrophoresis platform to confirm the identity of forensic samples from an aircraft accident site. Absolute allele identification was not achieved with this method, but it was found to be suitable for comparative analysis, as demonstrated by validation of the results and conclusions from capillary electrophoresis. The nine CODIS Short Tandem Repeat loci and a gender discrimination locus used in this study have a greater than 5E+07 matching probability suitable for small sample sizes.
Format:

PDF
Download URL:

https://rosap.ntl.bts.gov/view/dot/40488/dot_40488_DS1.pdf
File Type:

[PDF - 608.83 KB]
Collection(s):

FAA Civil Aerospace Medical Institute ; US Transportation Collection
Main Document Checksum:

urn:sha256:33cb33de7e521f10bb50a270899359df6d374a0c24937b97222d3643ce1425da

File Language:

English

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