Development of a Low Cost Portable Fluorometry Technology and Quantification of Cannabinoids in Body Fluids

Details

• Creators:
  Valentine, J. L. (Jimmie Lloyd), 1940- ; Bryant, P. J. ; Gutshall, P. L. ; Gan, O. H. M. ; Niu, B. H. C. ; Psaltis, P.
• Corporate Creators:
  University of Missouri--Kansas City
• Corporate Contributors:
  United States. National Highway Traffic Safety Administration
• Subject/TRT Terms:
  • Drug tests
  • Marijuana
  • Fluorometers
  • Saliva
• Publication/ Report Number:
  DOT-HS-804-009 ; NTIS-PB296836
• DOI:
  https://doi.org/10.21949/1524976
• Resource Type:
  Research Paper
• Geographical Coverage:
  United States
• Edition:
  Final report
• Corporate Publisher:
  United States. National Highway Traffic Safety Administration
• Description:
  Technology was developed for determining delta sup 9-tetrahydrocannabinol (I) and its major metabolite 11-nor-delta sup 9-tetrahydrocannabinol-9-carboxylic acid (II) in human blood plasma utilizing high pressure liquid chromatography (hplc)-ultraviolet (uv) detection. The methods were found to be capable of determining 10 ng of I or II in 1 ml of human plasma. A third type of assay method was developed for identifying in human saliva one of the major constituents of marijuana, cannabinol (III). Results indicated that III photolytically converted to a highly fluorescent compound in the absence of air. Using this technique, a limited study of marijuana smoker's saliva and saliva from non-marijuana smokers to which I and III had been added was conducted. This study demonstrated that 1 ng/ml of III can be detected in saliva. /Abstract from report summary page/
• Format:
  PDF
• Funding:
  DOT-HS-4-00968
• Download URL:
  https://rosap.ntl.bts.gov/view/dot/1197/dot_1197_DS1.pdf
• File Type:
  [PDF - 2.34 MB]
• Collection(s):
  NHTSA - Behavioral Safety Research
• Main Document Checksum:
  urn:sha256:4639c178e4429195ab98b77c73bada00e35f9b90f1d71f1e021ee21ecaeda1cd