Standardizing Environmental DNA Methodologies for Coho Salmon

Details

• Creators:
  Kinziger, Andrew ; Bjorkstedt, Eric ; Buchheister, Andre ; Henderson, Mark ; Bandy, Gavin ; Herman, Braden ; Shaffer, Jason
• Corporate Creators:
  Humboldt State University Sponsored Programs Foundation
• Corporate Contributors:
  California Department of Transportation
• Subject/TRT Terms:
  Conservation DNA Methodologies Ecosystems Environmental Impacts Error Analysis Fishes Methodology Salmon
• Resource Type:
  Tech Report
• Geographical Coverage:
  California ; United States
• Edition:
  Final Report 01/02/2020 – 12/31/2023
• Corporate Publisher:
  California. Department of Transportation
• Abstract:
  The California Department of Transportation (Caltrans) is mandated to conduct Federal Endangered Species Act (FESA) Section 7 and California Endangered Species Act (CESA) consultations for projects potentially impacting federally and state-listed species. Coho salmon (Oncorhynchus kisutch), listed under both FESA and CESA, require precise data on presence/absence, distribution, and abundance for effective consultation and associated mitigation. California is also home to many other listed aquatic species. Caltrans maintenance and project delivery actions may require mitigation even when fish aren’t detected because of the potential for listed species to be illusive, their population numbers are low, and because traditional survey methods are sometimes invasive or inefficient due to methodologies having impact to habitats and right to enter requirements. Surveys can also be time consuming, labor-intensive and costly, and require a permit for capturing and handling listed species. Therefore eDNA methods have the potential to simplify and expedite environmental review and permitting. Caltrans would benefit by incorporating eDNA into their survey tool kit. The establishment of a database of site occupancy for listed species because it could be used to conduct consultations and as a baseline for future maintenance and project delivery needs. Further, it may allow Caltrans to contribute to establishing population abundance and distribution data in the future, which would advance knowledge conservation of listed species.
• Format:
  PDF
• Funding:
  65A0762
• Collection(s):
  US Transportation Collection
• Main Document Checksum:
  urn:sha-512:7c669883c4d83d5ba6861ac04ab88d2c55c0818a9f079ad4102b9bb51877b809e4393749e57c77bb108428142096448332abe5a6edd3037f48da05fe10600444
• Download URL:
  https://rosap.ntl.bts.gov/view/dot/74442/dot_74442_DS1.pdf
• File Type:
  [PDF - 16.56 MB ]