Automated differential fluorometric analysis of norepinephrine and epinephrine in blood plasma and urine.

Fiorica, Vincent; Moses, Russell; Civil Aeromedical Institute

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Automated differential fluorometric analysis of norepinephrine and epinephrine in blood plasma and urine.

1971-04-01
By Fiorica, Vincent ; Moses, Russell

[PDF-1.00 MB]

English

Details

Details:

Creators:

Fiorica, Vincent ; Moses, Russell

Fiorica, Vincent ; Moses, Russell Less -
Corporate Creators:

Civil Aeromedical Institute
Corporate Contributors:

United States. Office of Aviation Medicine
Subject/TRT Terms:

[+]

Acetic Acid Adrenaline Amines Aviation Medicine Blood Analysis Blood Plasma--Analysis Carbon Catecholamines Centrifugal Force Centrifuges Chemical Analysis Chemical Compounds Chemical Tests And Reagents Chemistry, Analytic--Automation Chlorides Chlorine Chlorine Compounds Chromatographic Analysis Chromatography Distillation Distillation Apparatus Electromagnetic Waves Electromagnetic Waves By Wavelength Ethylenediamine Fatigue Fatigue (Physiological Condition) Ferrocyanides Fluorimeter Fluorimetry Fluorometers Human Subject Testing Hydrogen Hydroxides Inorganic Compounds Iron Compounds Light Measurement Mixtures Nitrogen Noradrenaline Organic Compounds Oxidation Oxygen Reagents Sodium Sodium Acetate Solution (Chemistry) Solutions (Chemistry) Standard Deviation Stress (Physiology) Thioglycolic Acid Urea Urine Urine--Analysis Water
Publication/ Report Number:

FAA-AM-71-15 ; DOT/FAA/AM-71/15

FAA-AM-71-15 ; DOT/FAA/AM-71/15 Less -
Resource Type:

Tech Report
Geographical Coverage:

United States
Edition:

OAM report.
Corporate Publisher:

Civil Aerospace Medical Institute
Abstract:

An automated fluorometric trihydroxyindole procedure is described for the measurement of norepinephrine (NE) and epinephrine (E) in blood plasma or urine. The method employs conventional techniques for isolation of the catecholamines by alumina column chromatography. Column eluates are analyzed in an AutoAnalyzer system incorporating two fluorometers. Differentiation of the amines is based on differences in the fluorescence characteristics of the lutines of NE and E.

The accuracy (as estimated by recoveries of added amines) and precision of the method are comparable to those reported for other trihydroxyindole techniques. Representative values for urinary excretion and for plasma levels of NE and E compare favorably with values stated in the literature.
Format:

PDF
Collection(s):

FAA Civil Aerospace Medical Institute US Transportation Collection
Main Document Checksum:

[+]

urn:sha256:08dbe6d9fb25cdac04407ccbd5539aa9b346e3c226f9e3a128a689d93d482a61
Download URL:

https://rosap.ntl.bts.gov/view/dot/20935/dot_20935_DS1.pdf
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Automated differential fluorometric analysis of norepinephrine and epinephrine in blood plasma and urine.

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