Fatigue and stress studies : an improved semi-automated procedure for flurometric determination of plasma catecholamines.

Fiorica, Vincent; United States. Office of Aviation Medicine

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Fatigue and stress studies : an improved semi-automated procedure for flurometric determination of plasma catecholamines.

1966-04-01
By Fiorica, Vincent

[PDF-491.21 KB]

English

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Details:

Creators:

Fiorica, Vincent
Corporate Creators:

United States. Office of Aviation Medicine
Subject/TRT Terms:

[+]

Absorption Acetates Acetic Acid Alumina Aluminum Oxide Amines Automation Blood--Analysis Blood Analysis Blood Plasma Catecholamines Chemical Analysis Chemical Reactions Chemical Tests And Reagents Chemistry, Analytic Ethylenediaminetetraacetic Acid Fatigue Fatigue (Physiological Condition) Ferrocyanides Fluorescence Fluorimetry Human Subject Testing Perchloric Acid Reagents Sodium Sodium Acetate Sodium Hydroxide Stress (Physiology) Vitamin C
Publication/ Report Number:

AM 66-6 ; DOT/FAA/AM-66/06

AM 66-6 ; DOT/FAA/AM-66/06 Less -
Resource Type:

Tech Report
Geographical Coverage:

United States
Corporate Publisher:

Civil Aerospace Medical Institute
Abstract:

A semiautomated technique is described for the estimation of total catecholamines in plasma by the trihydroxyindole procedure. The method utilizes conventional alumina-column chromatography for isolation of the amines. Catecholamine oxidation, tautomerization, and fluorescence detection are accomplished by an automated chemical system.

Recoveries from plasma of 0.01 and 0.02 micrograms total catecholamines/10-ml sample were 86.4% plus or minus 8.9% (SD) and 85.6% plus or minus 5.8% (SD), respectively. Total catecholamine level in resting human plasma, determined by the present technique, was 0.89 plus or minus 0.15 (SD) micrograms/l.
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PDF
Collection(s):

FAA Civil Aerospace Medical Institute US Transportation Collection
Main Document Checksum:

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urn:sha256:c3b7deaa02440b9a48f18ee4f970f7a998c3c66255f497d84ca2ea3c08a67c75
Download URL:

https://rosap.ntl.bts.gov/view/dot/20766/dot_20766_DS1.pdf
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Fatigue and stress studies : an improved semi-automated procedure for flurometric determination of plasma catecholamines.

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