Fatigue and stress studies : an improved semi-automated procedure for flurometric determination of plasma catecholamines.

Details

• Creators:
  Fiorica, Vincent
• Corporate Creators:
  United States. Office of Aviation Medicine
• Subject/TRT Terms:
  • Automation
  • Chemical analysis
  • Amines
  • Reagents
  • Chemical reactions
  • Alumina
  • Sodium
  • Acetates
  • Acetic acid
  • Sodium hydroxide
  More +
• Publication/ Report Number:
  AM 66-6 ; DOT/FAA/AM-66/06
• Resource Type:
  Tech Report
• Geographical Coverage:
  United States
• Corporate Publisher:
  Civil Aerospace Medical Institute
• Abstract:
  A semiautomated technique is described for the estimation of total catecholamines in plasma by the trihydroxyindole procedure. The method utilizes conventional alumina-column chromatography for isolation of the amines. Catecholamine oxidation, tautomerization, and fluorescence detection are accomplished by an automated chemical system. ; Recoveries from plasma of 0.01 and 0.02 micrograms total catecholamines/10-ml sample were 86.4% plus or minus 8.9% (SD) and 85.6% plus or minus 5.8% (SD), respectively. Total catecholamine level in resting human plasma, determined by the present technique, was 0.89 plus or minus 0.15 (SD) micrograms/l.
• Format:
  PDF
• Download URL:
  https://rosap.ntl.bts.gov/view/dot/20766/dot_20766_DS1.pdf
• File Type:
  [PDF - 491.21 KB]
• Collection(s):
  FAA Civil Aerospace Medical Institute ; US Transportation Collection
• Main Document Checksum:
  urn:sha-512:471260e4614cc57ef26a5be0c7101d8cca9a3af8f872469bb21d0261da1f6ea17fd5ff2a056d1f4673519de95e22d77cd65cecc90f9c191b29f56258bd571460