Utility of Capillary Blood for Gene Expression Studies [Data Management Plan]

Details

• Creators:
  Nicholson, Scott J.
• Corporate Creators:
  United States. Department of Transportation. Federal Aviation Administration. Civil Aerospace Medical Institute
• Corporate Contributors:
  United States. Department of Transportation. Federal Aviation Administration
• Subject/TRT Terms:
  • Data management plans
  • Blood
  • Blood analysis
  • Capillaries
  • Aviation safety
  • Air transportation crashes
  • Crash investigation
• DOI:
  https://doi.org/10.48321/D14851d9a3
• Resource Type:
  Data Management Plan
• Geographical Coverage:
  United States
• Edition:
  Data Management Plan
• Corporate Publisher:
  United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
• Abstract:
  Blood RNA is typically derived from blood tubes collected using standard venous phlebotomy practices. Venipuncture provides ample material for RNA extraction, but presents challenges when considering the time, labor, and acceptability among study participants. This study examines the utility of capillary blood collected through standard fingerstick practices as a replacement for venous blood as a source for blood RNA for RNA sequencing. Volunteer subjects will provide venous and fingerstick blood, and RNA from each sample will be extracted from each sample. Extracted RNA will be sequenced, and sequence data from each collection method will be compared to assess what differences exist between sample collection methods. If capillary blood RNA is determined to be a reliable source of gene expression data with equivalence to venous blood RNA, the methods developed in this study may be applied to future blood sample collections intended for RNA sequencing.
• Format:
  PDF
• Download URL:
  https://rosap.ntl.bts.gov/view/dot/73578/dot_73578_DS1.pdf
• File Type:
  [PDF - 39.34 KB]
• Collection(s):
  US DOT Public Access Data Management Plans ; FAA Civil Aerospace Medical Institute
• Main Document Checksum:
  urn:sha-512:d45c678bee351bad09e55e73e304ab1c1dcadb5ae84cbc565034bbb99bbfc7627d46dcdef1806464f2e35b9af4c453a559c3c73b116bd9fd8aca61ad399c48b9