Utility of Capillary Blood for Gene Expression Studies [Data Management Plan]

Nicholson, Scott J.; United States. Department of Transportation. Federal Aviation Administration. Civil Aerospace Medical Institute

doi:10.48321/D14851d9a3

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Utility of Capillary Blood for Gene Expression Studies [Data Management Plan]

[PDF-39.34 KB]

English

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Details:

Creators:

Nicholson, Scott J.
Corporate Creators:

United States. Department of Transportation. Federal Aviation Administration. Civil Aerospace Medical Institute
Corporate Contributors:

United States. Department of Transportation. Federal Aviation Administration
Subject/TRT Terms:

[+]

Air Transportation Crashes Aviation Safety Blood Blood Analysis Capillaries Crash Investigation Data Management Plans
DOI:

https://doi.org/10.48321/D14851d9a3
Resource Type:

Data Management Plan
Geographical Coverage:

United States
Edition:

Data Management Plan
Corporate Publisher:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Abstract:

Blood RNA is typically derived from blood tubes collected using standard venous phlebotomy practices. Venipuncture provides ample material for RNA extraction, but presents challenges when considering the time, labor, and acceptability among study participants. This study examines the utility of capillary blood collected through standard fingerstick practices as a replacement for venous blood as a source for blood RNA for RNA sequencing. Volunteer subjects will provide venous and fingerstick blood, and RNA from each sample will be extracted from each sample. Extracted RNA will be sequenced, and sequence data from each collection method will be compared to assess what differences exist between sample collection methods. If capillary blood RNA is determined to be a reliable source of gene expression data with equivalence to venous blood RNA, the methods developed in this study may be applied to future blood sample collections intended for RNA sequencing.
Format:

PDF
Collection(s):

US DOT Public Access Data Management Plans FAA Civil Aerospace Medical Institute
Main Document Checksum:

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urn:sha256:8adc53f6f8546a0440710926a03802a9a9283b64e521c098d7c1173bb66c0a43
Download URL:

https://rosap.ntl.bts.gov/view/dot/73578/dot_73578_DS1.pdf
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Utility of Capillary Blood for Gene Expression Studies [Data Management Plan]

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