Identification and Quantification of 22 Benzodiazepines in Postmortem Fluids and Tissues Using UPLC/MS/MS

Angier, Michael K.; Saenz, Sunday R.; Ritter, Roxane M.; Lewis, Russell J.; United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute

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Identification and Quantification of 22 Benzodiazepines in Postmortem Fluids and Tissues Using UPLC/MS/MS

2018-05-01
By Angier, Michael K. ; Saenz, Sunday R. ; Ritter, Roxane M. ; ...

[PDF-1000.52 KB]

English

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Creators:

Angier, Michael K. ; Saenz, Sunday R. ; Ritter, Roxane M. ; Lewis, Russell J.

Angier, Michael K. ; Saenz, Sunday R. ; Ritter, Roxane M. ; Lewis, Russell J. Less -
Corporate Creators:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Corporate Contributors:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Office of Aerospace Medicine
Subject/TRT Terms:

[+]

Benzodiazepines Blood Analysis Chemical Analysis Drug Effects Forensic Medicine Forensic Toxicology LC/MS/MS Narcotics Postmortem Toxicology
Publication/ Report Number:

DOT/FAA/AM-18/01
Resource Type:

Tech Report
Geographical Coverage:

United States
Edition:

Final
Corporate Publisher:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Abstract:

Benzodiazepines, a class of drugs known to cause central nervous system depression, are widely prescribed for a variety of different medical conditions such as anxiety, insomnia, and as a preoperative sedative in conjunction with anesthesia. In fact, four are listed in the top 100 most prescribed drugs. Although there are many medicinal benefits with benzodiazepines, there are many impairing side effects that can impact the ability to safely operate an aircraft. Therefore, the Federal Aviation Administration's Forensic Toxicology Research Team has developed and validated a comprehensive method for the analysis of 22 benzodiazepines in postmortem fluids and tissues. The newly developed method uses ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC/MS/MS) and a single step crash-and-shoot extraction. This new method reduces extraction time, significantly reduces sample volume, and eliminates derivatization steps necessary for commonly employed methods involving solid-phase extraction and GC/MS. For each of the analytes, the linear dynamic range encompasses sub-therapeutic to toxic concentrations. This method proved to be accurate and reproducible with control values not exceeding 20% of the target concentration. Analytes were stable over a 5-day period when stored at 4°C as well as on instrument, post extraction. Additionally, all analytes were stable after three freeze/thaw cycles. Ion suppression was evaluated in a variety of postmortem fluids and tissues. Although there was minimal suppression in fluids, there was suppression observed in tissues. However, this suppression affected the internal standard similarly; therefore, quantitative reliability was maintained. This newly developed UPLC/MS/MS method has been proven to be accurate and precise in the identification and quantification of 22 benzodiazepines in postmortem fluids and tissues.
Format:

PDF
Collection(s):

FAA Civil Aerospace Medical Institute
Main Document Checksum:

[+]

urn:sha256:f56355d4fefeebf355fcb7900837bc5fa1579e5bde7eb34b26bea66fd98b8100
Download URL:

https://rosap.ntl.bts.gov/view/dot/57204/dot_57204_DS1.pdf
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Identification and Quantification of 22 Benzodiazepines in Postmortem Fluids and Tissues Using UPLC/MS/MS

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