United States Department of Transportation
i
Measurement and Control Strategies for Sterol Glucosides to Improve Biodiesel Quality – Year 2
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2011-02-01
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Edition:Final Report: August 2009 - January 2011
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Abstract:This project had the objective of measuring trace compounds in biodiesel called sterol glucosides (SG) so strategies to reduce their concentration could be investigated. A MALDI-TOF-MS (matrix assisted laser desorption ionization time of flight mass spectrometry) analytical method for rapid evaluation of sterol glucosides has been developed and validated. Sitosteryl-glucoside, campesteryl-glucoside, and stigmasteryl-glucoside were identified in residues that temporarily shut down the Inland Empire biodiesel plant in Odessa, Washington. Standards were created by purification and recrystallization of these problematic residues. The standard’s purity was validated with mass spectrometry (MS) and nuclear magnetic resonance (NMR) measurements. Preparative methods are necessary to isolate the trace concentrations of acylated sterol glucosides (ASG) and SG from triglycerides and phospholipids in oilseed extracts. We have evaluated several preparative procedures, a modified silica gel chromatography procedure, and developed a size exclusion chromatography (SEC) preparative method for evaluation of glycolipids with an emphasis on SG. These preparative methods were evaluated using this MS analytical technique. SEC was selected as the best preparative procedure for evaluating tetrahydrofuran (THF) oilseed extracts. A winter canola seed sample (Brassica. napus L. cv. Amanda) was analyzed.
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