Identification of Sildenafil (Viagra™) and Its Metabolite (UK 103,320) in Six Aviation Fatalities

Johnson, Robert D.; Lewis, Russell J.

doi:10.21949/1404238

i

Identification of Sildenafil (Viagra™) and Its Metabolite (UK 103,320) in Six Aviation Fatalities

2006-02-01
By Johnson, Robert D. ; Lewis, Russell J.

File Language:

English

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Creators:

Johnson, Robert D. ; Lewis, Russell J.
Corporate Creators:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Corporate Contributors:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Office of Aerospace Medicine
Subject/TRT Terms:

Aircraft Accident Investigation Erectile Dysfunction Fatalities Ionization LC/MS/MS/MS Medical Treatment Medication Pharmacology Research Scientific Method Sildenafil Toxicology Viagra
Publication/ Report Number:

DOT/FAA/AM-06/3
DOI:

https://doi.org/10.21949/1404238
Resource Type:

Tech Report
Geographical Coverage:

United States
Corporate Publisher:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Office of Aerospace Medicine
Abstract:

During the investigation of aviation accidents, postmortem samples from victims are submitted to the Federal Aviation Administration’s Civil Aerospace Medical Institute for toxicological analysis. This report presents a rapid and reliable method for the identification and quantitation of sildenafil (Viagra® ) and its active metabolite, UK-103,320. This procedure utilizes sildenafil-d8 as an internal standard for more accurate and reliable quantitation. The method incorporates solid-phase extraction and LC/MS/MS and MS/MS/MS utilizing an atmospheric pressure chemical ionization ion trap mass spectrometer in the positive chemical ionization mode. Solid-phase extraction provided an efficient sample extraction yielding recoveries ranging from 79 – 88%. The limit of detection for sildenafil and UK-103,320 was 0.39 and 0.19 ng/mL, respectively. The linear dynamic range for both compounds was 0.78 – 800 ng/mL. The method was employed for the determination of sildenafil and UK-103,320 in postmortem fluid and tissue specimens collected from 6 fatal aviation accident victims. The current method proved to be simple, accurate, and robust for the identification and quantitation of sildenafil and UK-103,320 in postmortem fluids and tissues.
Format:

PDF
Collection(s):

FAA Civil Aerospace Medical Institute
Main Document Checksum:

urn:sha-512:385377b81a2fd489b83fae1aacd1d4178e93cc792de4647964ae563d1635b99ad3ab8cd6bd43ef8522e4e1b78600b5ad0d5f42640aac461bf1972d4c200937d1
Download URL:

https://rosap.ntl.bts.gov/view/dot/85512/dot_85512_DS1.pdf
File Type:

[PDF - 428.69 KB ]

File Language:

English

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