Assessment of RNA-seq Sample Preparation Methodology [Data Management Plan]

Munster, Susan K.

doi:10.48321/D1D046

i

Assessment of RNA-seq Sample Preparation Methodology [Data Management Plan]

2023-04-01
By Munster, Susan K.

File Language:

English

Details

Creators:

Munster, Susan K.
Corporate Creators:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Subject/TRT Terms:
DOI:

https://doi.org/10.48321/D1D046
Resource Type:

Data Management Plan
Geographical Coverage:

United States
Edition:

Data Management Plan
Contracting Officer:

Munster, Susan
Corporate Publisher:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Abstract:

This study was designed to designed to address concerns regarding effects of lab processing techniques on downstream results and also to determine an optimal combination of software programs to use to align genetic data and assess differential expression. Homogeneous lab samples were tested using two different purification methods and compared to unpurified samples to determine if either purification method had any impact on differential expression. Genetic data was also simulated with a known rate of differential expression. All samples were aligned and analyzed for differential expression using combinations of seven different alignment programs and nine different differential expression programs. Results were compared to determine which alignment program(s) and which differential expression program(s) provided optimal results in terms of accuracy, time required for processing and data storage footprint.
Format:

PDF
Funding:

6973GA-18-D-00110
Download URL:

https://rosap.ntl.bts.gov/view/dot/68208/dot_68208_DS1.pdf
File Type:

[PDF - 171.94 KB]
Collection(s):

US DOT Public Access Data Management Plans ; Federal Aviation Administration
Main Document Checksum:

urn:sha-512:ff7abf786e8d25cef0f7308a40868552a34798e62f5569c8847c35f7bf32c2dc32ff56cfcb52e1873826b1168b32ed1ae904bd1a305c49647018ef0326b09ba1

An Evaluation of the Downstream Effects of Purification Methods on RNA-Seq Differential Expression

Ribonucleic acid sequencing (RNA-Seq) is a valuable and commonly used technique to quantify the number of individual RNA transcripts within a sample. ...

Munster, Susan K. ;

Uyhelji, Hilary A.
RNA-Seq Alignment and Differential Expression Software Comparison

The twofold goals for this study were to determine an optimum choice for ribonucleic acid sequencing (RNA-Seq) alignment software and to determine whi ...

Munster, Susan K. ;

Nicholson, Scott J.

File Language:

English

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