Assessment of RNA-seq Sample Preparation Methodology [Data Management Plan]

Munster, Susan K.; United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute

doi:10.48321/D1D046

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Assessment of RNA-seq Sample Preparation Methodology [Data Management Plan]

2023-04-01
By Munster, Susan K.

[PDF-171.94 KB]

English

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Details:

Creators:

Munster, Susan K.
Corporate Creators:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Subject/TRT Terms:

[+]

Alignment Aviation Safety Blood Analysis Data Management Plans Methodology Representative Samples (Testing) Ribonucleic Acid Sequencing RNA-Seq Software Packages
DOI:

https://doi.org/10.48321/D1D046
Resource Type:

Data Management Plan
Geographical Coverage:

United States
Edition:

Data Management Plan
Contracting Officer:

Munster, Susan
Corporate Publisher:

United States. Department of Transportation. Federal Aviation Administration. Office of Aviation. Civil Aerospace Medical Institute
Abstract:

This study was designed to designed to address concerns regarding effects of lab processing techniques on downstream results and also to determine an optimal combination of software programs to use to align genetic data and assess differential expression. Homogeneous lab samples were tested using two different purification methods and compared to unpurified samples to determine if either purification method had any impact on differential expression. Genetic data was also simulated with a known rate of differential expression. All samples were aligned and analyzed for differential expression using combinations of seven different alignment programs and nine different differential expression programs. Results were compared to determine which alignment program(s) and which differential expression program(s) provided optimal results in terms of accuracy, time required for processing and data storage footprint.
Format:

PDF
Funding:

6973GA-18-D-00110
Collection(s):

US DOT Public Access Data Management Plans Federal Aviation Administration
Main Document Checksum:

[+]

urn:sha256:a43d205214157ce7412eb24dfad2135c64ed76c458fd139a4c8e6349de355cbb
Download URL:

https://rosap.ntl.bts.gov/view/dot/68208/dot_68208_DS1.pdf
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Assessment of RNA-seq Sample Preparation Methodology [Data Management Plan]

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