Enhancement of Drug Detection and Identification by Use of Various Derivatizing Reagents on GC-FTIR Analysis

Huffine, Edwin F.; Canfield, Dennis V.; Civil Aeromedical Institute

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Enhancement of Drug Detection and Identification by Use of Various Derivatizing Reagents on GC-FTIR Analysis

1992-07-01
By Huffine, Edwin F. ; Canfield, Dennis V.

[PDF-902.36 KB]

English

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Creators:

Huffine, Edwin F. ; Canfield, Dennis V.

Huffine, Edwin F. ; Canfield, Dennis V. Less -
Corporate Creators:

Civil Aeromedical Institute
Corporate Contributors:

United States. Office of Aviation Medicine
Subject/TRT Terms:

[+]

Amines Derivatization Drugs Forensic Medicine Forensic Toxicology Gas Chromatography Infrared Spectroscopy Phenylpropanolamine Sympathomimetic Agents Toxicology
Publication/ Report Number:

DOT/FAA/AM-92/25
Resource Type:

Tech Report
Geographical Coverage:

United States
Corporate Publisher:

Civil Aerospace Medical Institute
Abstract:

Phenylpropanolamine (PPA) is a relatively common non-prescription sympathomimetic amine. As such, it is frequently detected during forensic analysis. The presence of phenylpropanolamine can be confirmed by using Gas Chromatograph-Fourier Transform Infrared (GC-FTIR) spectrophotometry. One constraint of the GC-FTIR is the quantity of material required to obtain a suitable IR spectrum. If a drug is a relatively weak infrared absorber, several micrograms may be required in order to obtain a clear, reliable spectrum. While this amount of material may be readily available for some types of analysis, it can easily exceed the quantity of material available in the forensic toxicology setting.

One method that can be used to increase a drug's infrared absorption is to derivatize the drug with a polyfluorinated acid anhydride. Since carbonyl and carbon-fluorine bonds are strong infrared absorbers, molecules that possess such bonds have a heightened sensitivity to GC-FTIR analysis. Polyfluorinated acid anhydrides are capable of adding both carbonyl and carbon-fluorine bonds to drugs that possess either a hydroxyl, primary or secondary amine, or primary or secondary amide functional groups. Several derivatizing reagents were used and the extent to which they enhanced the identification of phenylpropanolamine were compared. Of the reagents studied, heptafluorobutyric acid anhydride (HFAA) produced the greatest increase in the phenylpropanolamine's sensitivity to GC-FTIR identification. Prior to derivatization, 1.8 micrograms of phenylpropanolamine was required for identification on the GC-FTIR, while only 0.032 micrograms of phenylpropanolamine was required after derivatization with HFAA.
Format:

PDF
Collection(s):

FAA Civil Aerospace Medical Institute US Transportation Collection
Main Document Checksum:

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urn:sha256:c21898ec2b64e435cd4cf77d827e10a843f5cbf3e2895e6ec7d6eda2a1442040
Download URL:

https://rosap.ntl.bts.gov/view/dot/21394/dot_21394_DS1.pdf
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Enhancement of Drug Detection and Identification by Use of Various Derivatizing Reagents on GC-FTIR Analysis

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